EFFECT OF ALOIN EXTRACT ON THE INCREASE OF FIBROBLAS CELL EXPRESSION ON HEALING OF WOUND WOUNDS OF HORSE WHITE RATS (Rattus norvegicus) BY THE AGING PROCESS
DOI:
https://doi.org/10.61942/msj.v1i4.92Keywords:
Aloin, fibroblast, wound healingAbstract
Motivation/Background: The wound healing of aging skin can be assisted by using aloin that increases fibroblast cells. The aim of this research is to determine the effect of aloin extract on increasing fibroblast cells in wound healing in male rats (Rattus norvegicus) with aging process The method research is a post-test control group design. The research sample use twenty-four of six months male rats (Rattus norvergicus) which were divided into 4 groups. Fibroblast assessment was carried out on days 7 and 14 of aloin treatment. Fibroblast data was processed using normality test and significance tests, carried out using the measured repeated test followed by Bonferonni test. The results is highest number of fibroblasts on day 7 was in the control group (+) treated with 10% povidone iodine. The highest number of fibroblasts on day 14 was in treatment group 2 which was given aloin. The statistical test Measure Repeated test said that there was no significant difference between the control and treatment groups p = 0.503 (p> 0.05). However, in the Bonferonni post hoc test, the value of p = 0.000 was obtained, which means that giving aloin can increase the number of fibroblast cells. The conclusions is aloin extract increased the number of fibroblast cells in wound healing of male rats (Rattus norvegicus) with aging process, which has the same effectiveness as povidone iodine
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Abdullah D, Yulhasfi Febrianto B, Purnama Dewi N, Teti Vani A, Chairal Ulfah F. The Effectiveness of 80% Kefir Gel Against The Overview The Number of Fibroblasts in Healing Cuts Mice (Mus Musculus). J Kesehat Prima [Internet]. 2022 Feb 1 [cited 2022 Feb 3];16(1):18 24. Available from: http://jkp.poltekkesmataram.ac.id/index.php/home/article/view/748
Ahmad Z, Damayanti. Penuaan Kulit : Patofisiologi dan Manifestasi Klinis. Berk Ilmu Kesehat Kulit dan Kelamin – Period Dermatology Venereol. 2018;30(03):208–15.
Amelia R, Abdullah D, Pratama Y, Purwati E. Antimicrobial Activity of Lactic Acid Bacteria Found in Dadiah on Disease-Causing Skin Infections. Indian J Forensic Med Toxicol [Internet]. 2021 Apr 13 [cited 2021 Nov 28]; Available from: https://www.researchgate.net/publication/351023566_Antimicrobial_Activity_of_Lactic_Acid_Bacteria_Found_in_Dadiah_on_Disease-Causing_Skin_Infections
Andri NR, Elyani H, Andriana D. Perbandingan Efek Perasan Lidah Buaya (Aloe Vera L.) Dengan Povidone Iodine 10% Terhadap Kadar Transforming Growth Factor-Beta (Tgf-Β) Serum Dan Jumlah Sel Fibroblas Jaringan Kulit Luka Sayat Pada Punggung Tikus Wistar Jantan. J Bio komplementer Med. 2019;6(3):1–11.
Blair MJ, Jones JD, Woessner AE, Quinn KP. Skin Structure-Function Relationships and the Wound Healing Response to Intrinsic Aging. Adv Wound Care. 2020;9(3):127–43.
Desjardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: An update. Regen Med. 2018;13(5):491–5.
Dewi nadia purnama, Abdullah D, Vani ade teti. Identifikasi Kuarsetin Dengan Teknik Klt Dan Hplc Pada Aloe Vera Barbadensis Miller Identification Of Quarcetine With Tlc And Hplc Techniques At Aloe Vera Barbadensis Miller | Request PDF [Internet]. Jurnal Kesehatan Medika Saintika. 2020 [cited 2022 Jan 11].
Dewi PS. Efektifitas ekstrak lidah buaya terhadap jumlah sel fibroblast pada proses penyembuhan luka incisi marmut. Intisari Sains Medis. 2018;9(3):51–4.
Donkor A, Ntiamoah M, Kuubabongnaa N. Akses terbuka Evaluasi potensi anti infeksi salep aloin A dan aloin yang diformulasi Terisolasi dari Aloe barbadensis Miller. 2020;1–8.
Donkor AM, Donkor MN, Kuubabongnaa N. Evaluation of anti-infective potencies of formulated aloin a ointment and aloin a isolated from Aloe barbadensis miller. BMC Chem. 2020;14(1):1–8.
Galomat DE, de Queljoe E, Datu OS. Pengaruh Pemberian Salep Ekstrak Daun Pegagang (Centella asiatica) (L.,) Urb. Terhadap Penyembuhan Luka Sayat Tikus Putih Jantan (Rattus norvegicus). Pharmacon. 2021;10(11):1205–14.
Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermatoendocrinol [Internet]. 2012 Jul [cited 2022 Feb 6];4(3):308. Available from: /pmc/articles/PMC3583892/
Kumar S, Yadav JP. Ethnobotanical and pharmacological properties of Aloe vera: A review. J Med Plants Res. 2014;8(48):1387–98.
Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci [Internet]. 2016 Oct 1 [cited 2022 Sep 21];73(20):3861–85. Available from: https://pubmed.ncbi.nlm.nih.gov/27180275/
Lee W, Yang S, Lee C, Kyun E, Kim K, Ku S, et al. Aloin reduces inflammatory gene iNOS via inhibition activity and p-STAT-1 and NF- κ B. Food Chem Toxicol. 2019;126(January):67–71.
Li LJ, Gao SQ, Peng LH, Wang XR, Zhang Y, Hu ZJ, et al. Evaluation of efficacy of aloin in treating acute trauma in vitro and in vivo. Biomed Pharmacother. 2017 Apr 1;88:1211 9.
Liang J, Cui L, Li J, Guan S, Zhang K, Li J. Aloe vera: A Medicinal Plant Used in Skin Wound Healing. Tissue Eng - Part B Rev. 2021;27(5):455–74.
Liu FW, Liu FC, Wang YR, Tsai HI, Yu HP. Aloin Protects Skin Fibroblasts from Heat Stress-Induced Oxidative Stress Damage by Regulating the Oxidative Defense System. PLoS One [Internet]. 2015 Dec 1 [cited 2022 Feb 6];10(12):e0143528. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143528
Lolou V, Panayiotidis MI. Functional Role of Probiotics and Prebiotics on Skin Health and Disease. Ferment 2019, Vol 5, Page 41 [Internet]. 2019 May 17 [cited 2021 Nov 28];5(2):41. Available from: https://www.mdpi.com/2311-5637/5/2/41/htm
Mahor G, Ali SA, Parveen N. Aloin From Aloe Vera Leaves: A Potential Natural Aluminium Detoxificant. Biosci Biotechnol Res Commun. 2019 Jun 25;12(2):531–8.
Marphirah M. Pemberian Salep Ekstrak Bunga Biduri (Calotropis gigantea) untuk Penyembuhan Luka pada Mencit (Mus musculus) Secara Klinis dan Histopatologis. Biot J Ilm Biol Teknol dan Kependidikan. 2019;6(2):139.
Nadia Purnama Dewi, Ade Teti Vani, Dessy Abdullah, Meta Oktora FM. Nusantara Hasana Journal. Nusant Hasana J. 2022;1(1):59–69.
Nida Sha , Rehana Mushtaq FB. Wound Healing Efficacy of Honey, Aloe Vera, And Turmeric Muhammad. 2021;4(c):37–40.
Putri Bunga Anggreno Setiawan B, Teti Vani A, Yulhasfi Febrianto B, Tri Septiana V, Bunga Anggreno Setiawan P. The Effectiveness of Using Aloe Vera Facial Soap and Aloe Gel on the Degree of Acne Vulgaris in Students of SMA Negeri 2 Bayang. J EduHealth [Internet]. 2020 Sep 20 [cited 2022 Mar 23];11(1):39–47. Available from: http://ejournal.seaninstitute.or.id/index.php/healt/article/view/151
Rahmayanti S, Satoto TBT. Fraksi Larut Metanol Kulit Lidah Buaya (Aloe Vera) Sebagai Larvasida terhadap Larva Nyamuk Aedes Aegypti (Diptera: Culicidae). 2021;181–8.
Renuga G. Formulation of Moisturizer Aloin from Aloe Vera Plant as Cosmetic Skin Protective Medicine. Int J Res Appl Sci Eng Technol. 2019 Feb 28;7(2):410–1.
Sari W., Berawi K., Karima N. Managemen Topikal Anti-Aging pada Kulit. Medula. 2019;9:237–43.
Sumbayak EM. Tinjauan Pustaka Fibroblas : Struktur dan Peranannya dalam Penyembuhan Luka. J Kedokt Meditek. 2015;21(6):1–6.
Vani ade teti. Gel Aloe Vera - Google Play Books [Internet]. 1st ed. Silpi M, Rahman D hasbi, Anggreno B, editors. Indramayu: Adab; 2022 [cited 2022 Feb 2]. Available from: https://play.google.com/books/reader?id=cmBZEAAAQBAJ&pg=GBS.PR2&lr=&printsec=frontcover
Yusharyahya SN. Mekanisme Penuaan Kulit sebagai Dasar Pencegahan dan Pengobatan Kulit Menua. eJournal Kedokt Indones. 2021;9(2):150.
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Copyright (c) 2023 Nadia Purnama Dewi , Monica Putri Vedora, Ade Teti Vani, Dessy Abdullah, Irwan Triansyah
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